THE PRESENCE AND LOCALIZATION OF HEAT SHOCK PROTEIN 70 IN RAT MAST CELLS
S. V. Shabelnikov, O. A. Bystrova, M. G. Martynova
Institute of Cytology RAS, St. Petersburg;
e-mail: heartdev@mail.cytspb.rssi.ru
Heat shock protein 70 (Hsp70) is considered not only as a cytosolic stress protein, but also as an extracellular
molecule with immunomodulatory and signaling functions that play a role in adaptation to stress on cellular
and systemic levels. The active involvement of mast cells in adaptation to stress may be associated with the presence
if Hsp70 in secretory granules. Using immunoelectron microscopy, we showed that Hsp70 localized in
secretory granules of rat pericardial and peritoneal mast cells. Localization of Hsp70 in rat perinoneal mast cells
isolated by centrifugation on Percoll was confirmed by immunoblotting. The proposed involvement of mast
cells in production of extracellular Hsp70 and possible functions of Hsp70 inside the mast cells granules are discussed.
Key words: mast cell, secretory granules, heat shock protein 70
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