IMAGING OF SURFACE CELL ANTIGENS ON THE TUMOR SECTIONS OF LYMPH NODES
USING FLUORESCENCE QUANTUM DOTS
E.P. Raphalovskaya-Orlovskaya,1 L.A. Gorgidze,1 A.A. Gladkin,1
S. M. Tauger,1 I. A. Vorobjev 1,2,3,*
1 National Research Center for Hematology, Moscow,
2 A. N. Belozersky Institute of Phisico-Chemical Biology, M. V. Lomonosov Moscow State University,
and 3 Chair of Cell Biology and Histology of Biological Faculty, M. V. Lomonosov Moscow State University;
* e-mail: ivorobjev@mail.ru
The usefulness of quantum dots for the immunofluorescent detection of surface antigens on the lymphoid
cells has been studied. To optimize quantum dots detection we have upgraded fluorescent microscope that allows
obtaining multiple images from different quantum dots from one section. Specimens stained with quantum
dots remained stable over two weeks and practically did not bleach under mercury lamp illumination during tens
of minutes. Direct conjugates of primary mouse monoclonal antibodies with quantum dots demonstrated high
specificity and sufficient sensitivity in the case of double staining on the frozen sections. Because of the high
stability of quantum dots’ fluorescence, this method allows to analyze antigen coexpression on the lymphoid tissue
sections for diagnostic purposes. The spillover of fluorescent signals from quantum dots into adjacent fluorescent
channels, with maxima differing by 40 nm, did not exceed 8 %, which makes the spectral compensation
is practically unnecessary.
Key words: immunohistochemical investigations, immunofluorescent method, quantum dots, differential diagnostics of lymphom
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