Tsitologiya  2012  54 (2) : 185–190

NUCLEUS DNA CONTENT MEASUREMENT METHODS FEATURES J.G. Pichugin,,3 K.A. Semianov,4 A.V. Chernyshev,2,3 I.G. Palchikova,3,5 L.V. Omelyanchuk,1,3,* V.P. Maltsev 2,3 1 Institute of Chemical Biology and Fundamental Medicine SB RAS, 2 Institute of Chemical Kinetics and Combustion SB RAS, Novosibirsk, 3 Novosibirsk State University, 4 Institute of Cytology and Genetics SB RAS and 5 Technological Design Institute of Scientific Instrument Engineering SB RAS, Novosibirsk; * e-mail: ome@mcb.nsc.ru We suggest a new theoretical method of the flow cytometry DNA histograms and apply it for Drosophila melanogaster imaginal discs cells. The model gives a possibility to determine the proportions of cells in G1, G2 (M) and S cell cycle phases. We show that the precision of G1 and G2 (M) DNA content measurements is limited by the precision of device zero signal arrangement. The usage of calculated device zero and dividing cells as the DNA content standards may improve the precision of DNA content measurements. We also compared the precisions of different DNA content methods and draw the conclusion that the current precisions of different methods are similar and lie within 2–6 % interval. Key words:  fluorescent flow cytometry, DNA content, cell cycle |  Back   |  Contents   |  Main  |