THE EFFECT OF GLUTOXIM ON Na+ TRANSPORT IN FROG SKIN: THE ROLE OF CYTOSKELETON
A.V. Melnitskaya, Z.I. Krutetskaya, O.E. Lebedev, S.N. Butov, N.I. Krutetskaya, V.G. Antonov
Chair of Biophysics of St. Petersburg State University;
e-mail: avm242@hotbox.ru
Using voltage-clamp technique, the possible role of the cytoskeleton in the effect of pharmacological analogue
of oxidized glutathione (GSSG), drug glutoxim, on Na+ transport in the frog Rana temporaria skin was investigated.
It was shown for the first time that preincibation of the skin with the microtubular disrupter, nocodazole,
actin filament disrupter, cytochalasin D or protein phosphatase PP1/PP2A inhibitor, calyculin A, significantly
decrease the stimulatory effect of glutoxim on Na+ transport. The data suggest the involvement of
microtubules and microfilaments in the regulatory effect of glutoxim on Na+ transport in frog skin and that reorganization
of actin filaments or microtubules leads to inhibition of stimulatory effect of glutoxim on Na+ transport
in frog skin epithelia.
Key words: glutoxim, transepithelial Na+ transport, cytoskeleton
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