THE INVOLVEMENT OF ACTIN CYTOSKELETON IN GLUTOXIM AND MOLIXAN EFFECT
ON INTRACELLULAR Ca2+-CONCENTRATION IN MACROPHAGES
L.S. Kurilova, Z.I. Krutetskaya, O.E. Lebedev, N.I. Krutetskaya, V.G. Antonov
Chair of Biophysics of St. Petersburg State University;
e-mail: cozzy@mail.ru
Glutoxim and molixan belong to new generation of disulfide-containing drugs with immunomodulatory,
hepatoprotective and hemopoetic effect on cells. Using Fura-2AM microfluorimetry, two structurally distinct
actin filament disrupters, latrunculin B and cytochalasin D, and calyculin A, which causes actin filaments condensation
under plasmalemma, we have shown the involvement of actin cytoskeleton in the intracellular
Ca2+-concentration increase induced by glutoxim or molixan in rat peritoneal macrophages. Morphological data
obtained with the use of rhodamine-phalloidine have demonstrated that glutoxim and molixan cause the actin
cytoskeleton reorganization in rat peritoneal macrophages.
Key words: peritoneal macrophages, glutoxim, molixan, intracellular Ca2+-concentration, actin filaments
| Back
| Contents
| Main |
