TWO SPREADING STATES OF MESENCHYMAL CELLS OF THE HUMAN EMBRYO IN VITRO
T.A. Krylova, L.V. Kukhareva, Yu.P. Petrov
Institute of Cytology RAS, St. Petersburg;
e-mail: takrylova@mail.ru
Spreading mesenchymal cells of human embryo on plastic and type I collagens (from rat, sheep and bull)
was studied. Spreading of the cells on collagens was stronger than that in the control but no differences between
the different collagens were revealed. The cell perimeter, the spreading coefficient and the cell projection area
on the substrate were used as morphometric parametres. The spreading of cells was monitored for 0.5—2 h after
plating. During the spreading both on plastic and on collagen, the groups of small cells were revealed as separate
subpopulations. As a whole, such cells comprehend 9 % of the cell population in the control and 2 % in experiment.
We assume that this cell type is associated with a special independent functional state of the cells that precedes
cell spreading.
Key words: cell area, cell perimeter, spreading coefficient, type I collagen, subpopulation
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