IMMUCYTOCHEMICAL IDENTIFICATION OF SYNAPTOTAGMIN 1, SYNTAXIN 1, N-TYPE Ca2+-CHANNEL AND NICOTINIC CHOLINORECEPTOR
IN MOTOR NEUROMUSCULAR JUNCTIONS OF EARTWORM SOMATIC MUSCLE LUMBRICUS TERRESTRIS
M.E. Volkov,1 E.M. Volkov,1,3 L.F. Nurullin 2
1 Kazan medical state university, Russia and 2 Institute of biochemistry and biophysics of Kazan Science center of Russian Academy Science;
3 e-mail: euroworm@mail.ru
The somatic muscle of earthworm contains myoneural synapses, forming clusters of "synaptic buttons". There the proteins syntaxin 1, synaptotagmin 1 and alpha 1Â subunit of the
Ca2+-channel of N-type were identified. It is supposed that "synaptic buttons" contain limited number of active zones that is associated with their small size (1-2 microns)
and the pattern of distribution of proteins of exo-endocytotic cycle. The postsynaptic membrane of cholinergic synapses contains nicotinic acetylcholine receptors capable to bind
alpha-bungarotoxin. The area of location of receptors on postsynaptic membrane is strictly limited to the region of synaptic contact.
Key words: myoneural synapses, syntaxin, synaptotagmin, Ca2+-channel, receptor to acetylcholine, earthworm
| Back
| Contents
| Main |
