HEAT SHOCK INDUCES SIMULTANEOUS REARRANGEMENTS OF ALL KNOWN CYTOSKELETAL FILAMENTS IN NORMAL INTERPHASE FIBROBLASTS
L.P. Gavrilova,1,* I.I. Korpacheva,1 S.G. Semushina,2 V.A. Yashin 3
1 Institute of Protein Research, Russian Academy of Sciences, 2 Branch of M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic
Chemistry, 3 Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia;
* e-mail: lpg@vega.protres.ru
We studied the cytoskeleton heat shock response (CHSR) in normal interphase embryonic (NERF) and postnatal rat fibroblasts (NPRF) using fluorescent microscopy.
Incubation of the cells at 43 °C resulted in reorganization of the cytoskeleton. CHSR in NERF and in NPRF were similar. Under heat shock, the bundles of actin filaments
disassembled, microtubule morphology changed remarkably, and intermediate filaments collapsed around the nucleus. After 30-120 minutes at 43 °C cells were still capable of
regaining the actin cytoskeleton after the temperature returned to normal (37 °C). Analysis of CHSR dynamics showed that rearrangements of different types of the cytoskeletal
filaments proceeded simultaneously, and they were visible after 2-4 minutes. We believe that the cytoskeleton reorganization under heat shock can be of vital importance in cell
protection against temperature stress. There are data about possible conjugation of CHSR with the rearrangement of the protein synthesizing system, which results in synthesis
of heat shock proteins.
Key words: cytoskeleton, heat shock, normal embryonic and postnatal fibroblasts, fluorescent microscopy
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