A COMPARATIVE STUDY OF FLUORESCENT PATTERNS IN THE NUCLEI OF EARLY MOUSE EMBRYOS USING ANTIBODIES AGAINST
DIFFERENT DOMAINS OF THE ACTIN MOLECULE
Irina O. Bogolyubova
Institute of Cytology, RAS, St. Petersburg;
e-mail: ibogol@mail.ru
In this work, new data on the peculiarities of actin immunofluorescent detection in 2-cell mouse embryos using antibodies against C- and N-terminal domains are presented.
We studied the distribution of nuclear actin after artificial suppression of transcription and after enzymatic digestion of DNA. The visual observations were supplemented with
morphometric analyses of confocal images. In both experimental groups (treated by transcription inhibitors or DNase) the reliable increasing of fluorescence intensity is revealed
when antibody against C-, but not against N-terminal domain of actin was used. These finding allows to suppose that antibody against C-terminal domain of actin is suitable with
a high efficiency for immunofluorescent studies on the nuclei of cleavage mouse embryo.
Key words: preimplantation mouse embryos, nuclear actin, transcription, laser scanning confocal microscopy
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