COMPARATIVE STRUCTURAL AND FUNCTIONAL CHARACTERISTICS OF DIFFERENT FORMS OF SACCHAROMYCES CEREVISIAE
RED PIGMENT AND ITS SYNTHETIC ANALOGUE
T.R. Amen,1 E. V. Mikhailova, 2,* V.V. Alenin,1 A.V. Artyomov,2 P.A. Dementyev,3
M.A. Khodorkovskii,4 T.O. Artamonova,4 I.M. Kyznetsova,2 T.R. Soidla,2 O.V. Nevzglyadova 2
1 Saint-Petersburg State University; 1 Institute of Cytology of RAS, St. Petersburg,
3 Ioffe Physical Technical Institute, RAS, St. Petersburg, and 4 St.Petersburg State Polytechnical University;
* e-mail: mikhailovaev@list.ru
Structural and functional characteristics of the yeast red pigment (product of polymerization of N1-(β-D-ribofuranosyl)-5-aminoimadazole),
isolated from ade1 mutant cells of Saccharomyces cerevisiae, its deribosylated derivatives (obtained by acid hydrolysis) and its synthetic pigment analogue
(product of polymerization of N1-methyl-5-aminoimidazole in vitro) were obtained. Products of in vitro polymerization were identified using mass
specrometry. The ability of these pigments to inhibit amyloid formation using insulin fibrils was compared. All the studied compound are able to interact with amyloids and
inhibit their growth. Electron and atomic force microscopy revealed a common feature inherent in the insulin fibrils formed in presence of these compounds - they are merged
into conglomerates more stable and resistant to the effects of ultrasound in comparison with insulin aggregates grown without pigments. We speculate that all these compounds
can cause coalescence of fibrils, partially blocking the loose ends and, thus, inhibit the attachment of the monomers and the formation of new fibrils.
Key words: insulin, amyloid, prion, Thioflavine T, red pigment, Saccharomyces cerevisiae, 5-aminoimidazoleridotide (AIR)
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