APOPTOSIS OF PERIPHERAL BLOOD LYMPHOCYTES IN PATIENTS
WITH LRRK2-ASSOCTATED PARKINSON’S DISEASE
T.S. Usenko,1,2 A.K. Emelyanov,1,2 A.F. Yakimovskii,2 N.A. Bogankova,3 T.V. Vavilova,3
A.L. Schwarzman,1 S.N. Pchelina 1,2,*
1 Petersburg Nuclear Physics Institute RAS, 2 St. Petersburg State I. P. Pavlov Medical University,
and 3 St. Petersburg State Medical Academy;
* e-mail: sopchelina@hotmail.com
Mutations in the Leucine Reach Repeat Kinase 2 (LRRK2) gene are the most frequent cause of familial Parkinson’s
disease (PD). Although the precise physiological and pathological role of LRRK2 is unclear, a direct
lin between mutant LRRK2 and apoptosis has been suggested. Using How cytometric analysis (PI+Annexin
V(FITC)) we showed increased spontaneous apoptosis of peripheral blood lymphocytes in patients with
LRRK.2-associated PD compared to controls after 24 (P < 0.016) and 48 (P < 0.031 ) h of incubation (5 % CO2,
37 °C). We found the increased FAS mRNA level in peripheral blood lymphocytes of patients with LRRK2-associated
PD compared to controls (P < 0.05) and to sporadic PD (sPD) (P < 0.002). Significant difference in
FAS expression between patients with LRRK2-associated PD and controls remained after three years and was
detected after 1 and 24 h during lymphocyte incubation (P < 0.03 and 0.05, respectively). Increased spontaneous
lymphocytes apoptosis along to increased FAS expression in patients with LRRK2-associated PD suggest that
LRRK2 mutations may lead to the activation of extrinsic apoptotic way.
Key words: apoptosis, BCL-2, FAS, LRRK2, lymphocytes, Parkinson’s disease
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