THE EFFECT OF NUCLEAR DYES ON THE FREQUENCY OF ABERRATIONS
IN MUCOSAL CELLS OF HUMANS
V.N. Kalaev, V.G. Artyukhov, M.S. Nechaeva
Department of Genetics, Cytology and Bioengineering of Voronezh State University;
e-mail: Dr_Huixs@mail.ru
The effect of staining with commonly used nuclear dyes (orsein, light green, methylene blue, azure-eosin
by Romanovsky—Giemsa) on the frequency of occurrence of nuclear disorders (micronuclei, perinuclear vacuoles,
notches, protrusions, such as «tongue» and «broken egg») in human buccal mucosa was determined. It is
shown that Romanovsky—Giemsa dye is the best type of dye both in the quality of the micropreparations and the
spectrum of detected abnormalities.
Key words: buccal mucosa, nuclear dyes, micronuclei, perinuclear vacuoles, notches, protrusions, such
as «tongue» and «broken egg»
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