BIOLOGICAL AND MORPHOLOGICAL CHARACTERIZATION OF IN VITRO
EXPANDED HUMAN MUSCLE-DERIVED STEM CELLS
L. Danišovič,1, * I. Varga,2 S. Polák Š.,2 B. Bajčikova,3
M. Adamkov,4 J. Vojtaššák 1
1 Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine,
Comenius University in Bratislava, Slovakia,
2 Institute of Histology and Embryology, Faculty of medicine, Comenius University in Bratislava, Slovakia,
3 Department of Vascular Surgery, University hospital, Bratislava, Slovakia,
and 4 Institute of Histology and Embryology, Jessenius Faculty of Medicine in Martin,
Comenius University in Bratislava, Martin, Slovakia;
* e-mail: lubos.danisovic@fmed.uniba.sk
Stem cells are generally characterised as clonogenic and undifferentiated cells with the capacity of self-renewal
and plasticity. Over the past few years, the adult stem cells have been derived from various types of tissues
including the skeletal muscle. The main goal of the present study was the isolation, in vitro expansion and
characterisation of muscle-derived stem cells (MDSCs). Thereby obtained results showed that MDSCs have a
fibroblast-like shape with a large nucleus having one to four nucleoli. The cytoplasm was transparent without
any signs of vacuolisation. TEM analysis showed an ultrastructure of cells with high proteosynthetic activity.
MDSCs had a large and irregular nucleus with variable number of nucleoli. The cytoplasm contained a richly
developed and rough endoplasmic reticulum, prominent Golgi apparatus cisterns as well as transport vesicles
containing glycogen granules and variable microvilli and filopodia. They expressed alpha-actin and desmin. Results
of the phenotypic characterization showed that the analyzed cells were positive for CD29, CD34, CD44,
CD90, CD105 and HLA Class I. They did not express CD14, CD45, CD235a, HLA Class II and human fibroblast
surface protein. According to these results it should be emphasised that MDSCs after performing the detailed
studies focused on their immunological properties and differentiation potential may be used in the cell therapy
of many degenerative diseases.
Key words: muscle-derived stem cells (MDSCs), isolation, cell culture, morphological analysis, immunophenotyping
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