EFFECT OF ACETYLCHOLINE AND ACETYLCHOLINESTERASE ON THE ACTIVITY
OF CONTRACTILE VACUOLE OF AMOEBA PROTEUS
Ya. Yu. Bagrov, N. B. Manusova 1
I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry RAS, St. Petersburg;
1 e-mail: nmanus@rambler.ru
Acetylcholine (ACh, 1 ìM) stimulates activity of the contractile vacuole of proteus. The effect of ACh is
not mimicked by its analogs which are not hydrolyzed by acetylcholinesterase (AChE), i. e., carbacholine and
5-methylfurmethide. The effect of ACh is not sensitive to the blocking action of M-cholinolytics, atropine and
mytolone, but is suppressed by N-cholinolytic, tubocurarine. The inhibitors of AChE, eserine (0.01 ìM) and armine
(0.1 ìM), suppress the effect of ACh on amoeba contractile vacuole. ACh does not affect activation of
contractile vacuole induced by arginine-vasopressin (1 ìM), but it blocks such effect of opiate receptors agonist,
dynorphin A1-13 (0.01 ìM). This effect of ACh is also suppressed by the inhibitors of AChE. These results suggest
that, in the above-described effects of ACh, AChE acts not as an antagonist, but rather as a synergist.
Key words: protozoa, Amoeba proteus, contractile vacuole, acetylcholine, acetylcholinesterase, acetylcholinesterase
non-enzymatic effect, arginin-vasopressin, dynorphin A (1-13)
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