ACTIVATION OF TRANSCRIPTION FACTOR NF-kB BY CARCINOGENIC POLYCYCLIC
AROMATIC HYDRICARBONS
M. S. Volkov, V. A. Kobliakov
Institute of Carcinogenesis, N. N. Blokhin Cancer Research Centre RAMS, Moscow;
e-mail: kobliakov@rambler.ru
Effect of carcinogenic polycyclic aromatic hydrocarbons (PAH) benzo(a)pyrene (BP) and 3-methylcholanthrene
(MC) on transcription factor NF-kB activation was studied. The determination of NF-kB activity was performed
by two different methods: determination of mRNA expression of NF-kB-dependent I-kB gene, and determination
of transcription activity of co-transfected with the plasmid containing the luciferase reporter gene
under the NF-kB-sensitive promoter. As a subject of inquiry the hepatoma cell cultures HepG2 expressed Ah receptor
and G27 not expressed Ah receptor were used. BP and MC weekly enhanced NF-kB activity in proliferating
HepG2 cells. The enhance of NF-kB activity was significantly higher in resting cells. NF-kB activation by
BP and MC in hepatoma G27 cells was significantly higher in hepatima G27 cells than in HepG2 cells both in
proliferating and resting cells. The role of Ah receptor in PAH action on NF-kB activation is discussed.
Key words: Ah receptor, carcinogenesis, NF-kB, polycyclic aromatic hydrocarbons
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