RESEARCH OF BIOCOMPATIBILITY OF NANOPARTICLES WITH FLUORESCENT DOMEN Er/Yb
IN THE NEUTROPHILIC GRANULOCYTES SYSTEM
S. N. Pleskova,1, 2, * E. N. Gorshkova,1 E. R. Mikheeva,1, 2 A. N. Shushunov 1
1 Research and Education Center for «Physics of solid state nanoctructures»
N. I. Labachevsky State University of Nizhni Novgorod and 2 Department of Biotechnologies, Physical and Analytical Chemistry R. Y. Alekseev
Nizhni Novgorod State Technical University;
* e-mail: pleskova@mail.ru
Fluorescent tags are extensively used for the diagnostic, labeling and marking in vivo and in vitro systems.
In this study, fluorescent nanoparticles with Er/Yb lightning center were tested on neutrophilic granulocytes.
The main purpose was to idenfity possible toxic effect. The negative impact of fluorophores on the metabolism
of neutrophilic and their enzyme systems, on the receptor-mediated cell responses and on the rigidity of cell
membranes was shown. The viability of neutrophils (estimated with the use of propidium iodide) after 2 hours
of incubation with the fluorescent nanoparticles in concentrations of 10–4 and 10–3 mM was 27.0 ± 6.6 and
19.07 ± 3.34 %, respectively.
Key words: nanosized fluorophores, neutrophils granulocytes, viability
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