NADP INCREASES THE LEVEL OF HISTONE H2AX PHOSPHORYLATION IN MOUSE HEART CELLS AFTER IONIZING RADIATION
D. V. Firsanov,1 A. V. Kropotov, V. M. Mikhailov
Institute of Cytology RAS, St. Petersburg;
1 e-mail: dfirsanov@gmail.com
Phosphorylation of replaceable histone H2AX occurs in megabase chromatin domains around DNA double-strand breaks (DSBs), and this modification called γ-H2AX
can be used as an effective marker for DSBs repair and DNA damage response. Using Western blotting and immunohistochemistry techniques we have studied here the influence
of exogenous nicotinamide adenine dinucleotide phosphate (NADP) which could potentially increase the intracellular level of NAD+ and on the level of γ-H2AX formation in
mouse heart cells after ionizing radiation (IR). We have found that injection of NAD+ in different doses immediately after IR causes an increased level of γ-H2AX in mouse
heart cells 20 min after IR at the dose of 3 Gy compared to control mice after IR exposure. It indicates that it could be a relationship between intracellular NAD+ content and
DNA damage response in vivo.
Key words: ionizing radiation, histone H2AX phosphorylation, NADP, DNA damage response
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