DNA REPEATED SEQUENCES MAY BE INVOLVED IN THE SYNAPTONEMAL COMPLEXES FORMATION
V. E. Spangenberg,1 S. Y. Dadashev, O. L. Kolomiets, Yu. F. Bogdanov
N. I. Vavilov Institute of General Genetics RAS, Moscow;
1 e-mail: vspangenberg@gmail.com
Synatonemal complexes (SCs) are the intranuclear structures which facilitate reversible lateral synapsis of the homologous chromosomes in the course of meiosis. It is still unclear which
DNA nucleotide sequences are responsible for the chromatin attachment to the SC lateral elements. Considering the features of the dispersed re-peated sequences (RS) it is worth to assume
their participation in the structure functional organization of the meiotic chromosome. Using numerical analysis we have investigated the relationship between RS and the distribution of events
of the meiotic recombination in mouse chromosome 1. Using in situ hybridization on spread mouse spermatocytes, we have demonstrated the arrangement of different types of RS
relative to SCs. Hybridization signals of B1(Alu), B2, and minisatellite probes were localizating predominantly in the SCs regions. Our results allow us to suggest the model of the meiotic
chromosome organization with the RS as the sequences, participating in the attachment of chromatin loops and SCs.
Key words: synaptonemal complex, DNA, chromosome organization, meiosis, SINE, LINE
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