CHANGE IN LOCALIZATION OF CELLULAR VESICULAR APPARATUS DURING DIFFERENTIATION OF MYOBLASTS INTO MYOTUBULES IN CELL CULTERE
A. V. Salova, E. A. Leontieva, T. P. Mozhenok, E. S. Kornilova, S. A. Krolenko, T. N. Belyaeva
Institute of Cytology RAS, St. Petersburg;
e-mail: asalova@gmail.com
The study of changes in the intracellular processes during differentiation of myoblasts into myotubules is of great importance for understanding several fundamental problems of cell biology.
At first, this concerns the spatial organization of vacuolar apparatus that reflects the alterations in the properties of cell membranes, cytoskeleton elements and dynamics of vesicular transport
in the course of differentiation. The distribution of acidic membrane organelles (lysosomes, late endosomes, Golgi cisternae) during the myotubule formation was revealed. It was shown that
perinuclear localization of acidic organelles in myoblasts was replaced by diffuse distribution of these structures in the whole volume of myotubules. Using lipophilic fluorescent dyes, RH 414
and di-8-ANEPPS, the process of formation and dynamics of endocytic vesicles in myoblasts and myotubules was investigated. In the present work, semiconductive nanocrystals, quantum
dots (QDs), conjugated with TAT-peptide, which belongs to cell-penetrating peptides, were used to characterize nonspecific endocytosis. It was shown that QDs-TAT complexes penetrate
myoblasts but do not penetrate myotubules even after 24 h incubation, which might be connected with plasma membrane changes during the process of skeletal muscle differentiation.
Key words: myoblasts, myotubules, differentiation, quantum dots, confocal microscopy, acridine orange, RH 414
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