NON-HISTONE SKELETON OF MITOTIC CHROMOSOME IN SITU
M. S. Makarov, Yu. S. Chentsov
M. V. Lomonosov Moscow State University, Biology Department;
e-mail: mcsimmc@yandex.ru,
yuchentsov@mail.ru
Studying giant nuclei of Chironomus plumosus in situ (Makarov, Chentsov, 2010), we concluded that po-lythene chromosome structure appears after 2 M NaCl and
DNase treatment in presence of 2 mM CuCl2. Cu2+-ions may stabilize bonds between specific non-histone components, arranged into non-histone matrix of
polythene chromosome. Here, we investigated the non-histone matrix of pig embryo mitotic chromosomes in situ, using 2 mM CuCl2-stabilization method. In 2 mM
CuCl2 - stabilized cells the residual chromosome body (non-histone matrix) could be visualized in every stage of mitosis. Mitotic chromosome non-histone matrix had the same
reaction on preliminary hypotonic treatment as normal chromosome: different decondensation of non-histone material was observed. Topoisomerase IIalpha and SMC 1 had uniform localization
inside chromosomal body and did not form any axial structures.
Key words: non-histone proteins, nuclear protein matrix, stabilization, topoisomerase IIalpha, SMC 1
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