NATIVE ELECTROPHORESIS IN CELL PROTEOMICS: BN-PAGE AND CN-PAGE
S. A. Shukolyukov
I. M. Sechenov Institute for Evolutionary Physiology and Biochemistry RAS, St. Petersburg;
e-mail: tinycapers@mail.ru
The presented mini-review aims to attract the attention of domestic researchers for rapid, cheap and easily reproducible method of native polyacrylamide gel electrophoresis
(PAGE), which for some reason has not yet found application in our country. The review collected the most interesting examples of the use of three types of native
electrophoresis (BN-PAGE, CN-PAGE and hrCN-PAGE) to study the peculiarities of proteomes of various animal, plant and bacterial cells. The references to fundamental
reviews, basic protocols, modifications of the initial methods and the examples of the combination of native electrophoresis with other chemical or physical methods are
presented. Particular attention to the principles of BN-, CN- and hrCN-PAGE as well as to their advantages and disadvantages is paid.
Key words: native electrophoresis, BN-PAGE, CN-PAGE, hrCN-PAGE, detergents, Comassie G-250
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