THE CELL CYCLE REGULATOR P130 AND β-CATENIN FORM A COMPLEX IN MESENCHYMAL STEM CELLS
N. S. Petrov, O. V. Zhidkova, V. V. Zenin, Y. M. Rosanov, B. V. Popov 1
Institute of Cytology RAS, St. Petersburg;
1 e-mail: avg@main.vsu.ru
Suppressor complex p130/E2f4 inhibits transcription of multiple genes proteins regulating cell cycle progression and induces cell cycle arrest at
G0/G1 required for induction of cell differentiation in cells of many tissues in vivo and various cell lineages in vitro. We found here that, in
mesenchymal stem cells, (MSC) activation of the Wnt/â-catenin signal pathway induced by MSC coculture with the A-549 cell line or by growth in the medium containing
Li+ ions, which resulted in the accumulation of active forms of the p130, E2f4 and β-catenin, was not coupled with inhibition of cell cycle progression.
Cell cycle synchronization of the MSC induced by thymidine and nocodazol was not resulted in change of the levels and phosphorylation pattern of the p130 in contrast to
mouse hepatocytes and T98G cells which showed accumulation of the p130 form p1 and p2 in quiescence and form p3 under active proliferative. Antibody to p130 precipitated
from extracts of MSC activated by Li+ ions â the p130 form 2 and hyperphosphorilated β-catenin. The results obtained suggest that Gsk3β,
p130 and β-catenin form in MSC a complex the functional role of which may be associated with activation of differentiation not coupled to cell cycle arrest.
Key words: mesenchymal stem cells, p130, Wnt/β-catenin, E2f4, cell cycle, differentiation
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