STUDY OF CELL CULTURE OF MOUSE SUBMANDIBULAR SALIVARY GLAND IN VITRO
I. G. Gvazava, A. V. Vasiliev, O. V. Balan, V. V. Terskikh
N. K. Koltzov Institute of Developmental Biology RAS, Moscow;
e-mail: inessagvazava@hotmail.ru
Cell culture keeping its phenotype till the 20th passage was obtained from mouse submandibular salivary glands. The analysis of the heterogeneous culture showed that
there were some morphological types of cells: densely packed small cells that had cuboidal or polygonal form and also large round cells. Epithelial cells of submandibular
gland cultured during several weeks were able to form tubular strucures. It was shown that glan-dulocyte culture was presented by K19-positive and NGF-positive cells.
It is important to note that expression of genes coding proinsulin and insulin was detected by immunocytochemical staining and by PCR as well.
Key words: submandibular salivary glands, mouse, population of glandulocytes in culture, proinsulin, insulin
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