DYNAMICS OF MATRIX METALLOPROTEINASES ACTIVITY OF PRIMARY MURINE
EMBRYONIC FIBROBLASTS DURING CULTIVATION
I. V. Voronkina, K. M. Kirpichnikova, L. V. Smagina, I. V. Kozhucharova, I. A. Gamaley 1
Institute of Cytology RAS, St. Petersburg;
1 e-mail: igamaley@mail.cytspb.rssi.ru
Embryonic cells regulate the expression of matrix metalloproteinases (MMP) providing remodulation of
extracellular matrix, which in turn provides the changes in cell adhesion and migration during the cell development
and differentiation. In present work we studied the changes of gelatinases (MMP-2 and MMP-9) and collagenases
(MMP-1 and MMP-8) activities in the process of cultivating the primary murine embryonic fibroblasts
(MEF). Cultivation was continued for 6 passages, after that the culture died in time. According to gelatin and
collagen zymography results, drastic changes of all MMPs activities occurred during the third passage of cell
cultivation. The MMP-1 and MMP-9 activity appears in the middle of cultivation and then disappeared at the
end. The most important event MEF cultivation is appearance and subsequent reservation of collagenase
MMP-8 and active form of gelatinase MMP-2.
Key words: gelatinases, collagenases, primary murine embryonic fibroblasts
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