2011  53 (1) : 17–21
COMPARATIVE ESTIMATION OF MORPHOFUNCTION CHARACTERISTICS ALIVE AND FIXED ERYTHROCYTES

M. Yu. Skorkina,1 M. Z. Fedorova, S. D. Chernyavsky, N. A. Zabinyakov, E. A. Sladkova

Department of Biotechnology, Morphology and Physiology of Alive Organisms of the Belgorod State University;
1 e-mail: skorkina@bsu.edu.ru

The method of power spectroscopy carries out the quantitative analysis of elastic properties of alive cells. It has been established that the highest indicators of elasticity nuclear (amphibious) and denuclearized (mammals) alive erythrocytes are registered in epi- and perinuclear space. When fixing with methanol and drying of cells the greatest values of the elastic modulus are displaced to the periphery of the cells. The revealed inversion of elasticity properties of erythrocytes must be considered when assessing the morphofunction characteristics of the fixed cells.

Key words:  cell, erythrocytes, morphofunction parameters, AFM-measurements, elastic-properties, module Yung


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