DISTRIBUTION OF INDIVIDUALS BY SPONTANEOUS FREQUENCIES OF LYMPHOCYTES
WITH MICRONUCLEI. PARTICULARITY AND CONSEQUENCES
A. M. Serebryanyi,1, * A. V. Akleyev,2 A. V. Aleshchenko,1 M. M. Antoschina,3
O. V. Kudryashova,4 N. I. Ryabchenko,3 L. P. Semenova ,1 I. I. Pelevina 4
1 Emanuel Institute of Biochemical Physics RAS, Moscow,
2 Urals Research Center for Radiation Medicine, Chelyabinsk,
3 Medical Radiological Research Center RAMN, Obninsk, and
4 N. N. Semenov Institute of Chemical Physics RAS, Moscow;
* e-mail: amserebr@sky.chph.ras.ru
By micronucleus (MN) assay with cytokinetic cytochalasin B block, the mean frequency of blood lymphocytes
with MN has been determined in 76 Moscow inhabitants, 35 people from Obninsk and 122 from Chelyabinsk
region. In contrast to the distribution of individuals on spontaneous frequency of cells with aberrations,
which was shown to be binomial (Kusnetzov et al., 1980), the distribution of individuals on the spontaneous frequency
of cells with MN in all three massif can be acknowledged as log-normal (χ2 test). Distribution of individuals
in the joined massifs (Moscow and Obninsk inhabitants) and in the unique massif of all inspected with great
reliability must be acknowledged as log-normal (0.70 and 0.86 correspondingly), but it cannot be regarded as
Poisson, binomial or normal. Taking into account that log-normal distribution of children by spontaneous frequency
of lymphocytes with MN has been observed by the inspection of 473 children from different kindergartens
in Moscow we can make the conclusion that log-normal is regularity inherent in this type of damage of lymphocytes
genome. On the contrary the distribution of individuals on induced by irradiation in vitro lymphocytes
with MN frequency in most cases must be acknowledged as normal. This distribution character points out that
damage appearance in the individual (genomic instability) in a single lymphocytes increases the probability of
the damage appearance in another lymphocytes. We can propose that damaged stem cells lymphocyte progenitor's
exchange by information with undamaged cells – the type of the bystander effect process. It can also be
supposed that transmission of damage to daughter cells occurs in the time of stem cells division.
Key words: lymphocytes, micronucleus assay, distribution of individuals on the spontaneous frequency of cells with micronucleus
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