OBSERVATION OF CELLS TOLERANT OF TOBACCO MOSAIC VIRUS
IN VIRUS-INDUCED LOCAL LESIONS IN DATURA STRAMONIUM L. LEAVES
A. V. Reunov,1 S. N. Lega, V. P. Nagorskaya, L. A. Lapshina
Pacific Institite of Bioorganic Chemistry, Far East Branch of RAS, Vladivostok;
1 e-mail: antreunov@mail.ru
Ultrastructural examination of tobacco mosaic virus-induced local lesions developing in leaves of Datura stramonium
plants demonstrated that, in the central area of the lesions, the cell response to viral invasion was
not uniform. Most cells exhibited an acute hypersensitive reaction and underwent rapid and complete necrosis.
However, some cells, despite considerable virus accumulation and immediate contact with completely collapsed
cells, maintained a certain degree of structural integrity. Analysis performed showed that the proportion of collapsed
and uncollapsed cells in the lesion centre 3 to 5 days after infection did not change essentially. These data
suggest that the absence of hypersensitive response in some cells in the lesion centre is not due to an early stage
of infection but is likely caused by cell tolerance of the virus.
Key words: Datura stramonium leaves, tobacco mosaic virus, hypersensitive reaction, tolerance, ultrastructure
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