THE EFFECT OF LOW TEMPERATURES ON THE VIABILITY OF HUMAN EPIDERMAL
KERATINOCYTES FOUND AT DIFFERENT STAGES OF DIFFERENTIATION
M. Raydan,1, * N. A. Shubin,1 M. I. Blinova,1, 2 G. G. Prokhorov,1 G. P. Pinaev 1
1 Institute of Cytology RAS and 2 International Institute of Cryomedicine, St. Petersburg;
* e-mail: raydanmazen@yahoo.com
The aim of this study was a comparative analysis to the degree of stability of human epidermal cells found
at different stages of differentiation to low temperatures. The effect of different subzero temperatures of liquid
nitrogen vapor on keratinocytes found both in human skin fragments and as isolated cells extracted from skin
fragments has been studied. The degree of stability of epidermal cells low temperatures was evaluated by their
ability to form a multilayer stratum in culture; hence this phenomenon explains the survival of a sufficient amount
of proliferative cells after exposure to subzero temperatures. Quantitative analysis of the ratio of epidermal
stem, transitory and differentiated cells in a population of viable cells before and after exposure to low temperatures
were determined using antibodies corresponding to their different stages of differentiation. The results of
this research show that the stability of human epidermal cells to low temperature differs depending on their stage
of differentiation both in situ and in vitro. Epidermal stem cells and transitory cells are more stable than differentiated
cells.
Key words: differentiated, stem cell and transitory keratinocytes, human epidermal cells, low temperatures
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