FLUOROMETRIC IDENTIFICATION OF CHROMATIN PACKING LEVEL LAYING OUT
OF THE LIGHT MICROSCOPY RESOLUTION
L. I. Lebedeva,1 T. D. Dubatolova,1 L. V. Omelyanchuk 1, 2
1 Institute of Chemical Biology and Fundamental Medicine SB RAS Novosibirsk, and
2 Novosibirsk State University;
e-mail: ome@mcb.nsc.ru
Confocal microscopy permits to perform quantitative analysis of the fluorescent signals of the nuclei. We
determined the level of DAPI and phosphorylated histone-H3 fluorescence, the volume of DAPI and phosphorylated
histone-H3 fluorescence in normal (Hikone AW) and colchicine treated third instar Drosophila melanogaster
wing imaginal disc mitotic cells. Our analysis permitted to indentify two unknown levels of chromatin package;
one in the prometaphase and another at the end of metaphase. These levels disappeared in colchicine treated
mitoses. We conclude that quantitative analysis of confocal images is able to detect the differencies in
chromatin package laying over the resolution of light microscopy.
Key words: confocal microscopy, fluorescence, H3-p-histone, DAPI, Drosophila melanogaster, imaginal disc
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