MOLECULAR COMBING METHOD IN THE RESEARCH OF DNA REPLICATION PARAMETERS
IN ISOLATED ORGANS OF DROSOPHYLA MELANOGASTER
A. V. Ivankin, T. D. Kolesnikova, S. A. Demakov, O. V. Andreenkov, E. R. Bildanova,
N. G. Andreenkova, I. F. Zhimulev
Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk;
e-mail: anton.ivankin@gmail.com
Methods of physical DNA mapping and direct visualization of replication and transcription in specific regions
of genome play crucial role in the researches of structural and functional organization of eukaryotic genomes.
Since DNA strands in the cells are organized into high-fold structure and present as highly compacted
chromosomes, the majority of these methods have lower resolution at chromosomal level. One of the approaches
to enhance the resolution and mapping accuracy is the method of molecular combing. The method is based
on the process of stretching and alignment of DNA molecules that are covalently attached with one of the ends
to the cover glass surface. In this article we describe the major methodological steps of molecular combing and
their adaptation for researches of DNA replication parameters in polyploidy and diploid tissues of Drosophyla
larvae.
Key words: molecular combing, replication, polyploid tissues, Drosophyla
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