FRAGMENTATION OF DNA LYMPHOCYTES THE HUMAN IN DYNAMICS OF DEVELOPMENT APOPTOSIS, INDUCED INFLUENCE OF UV-RADIATION
AND REACTIVE OXYGEN SPECIES
V. G. Artyukhov,1 M. S. Trubicina, M. A. Nakvasina, E. V. Solovjeva
Voronezh State University;
1 e-mail: avg@main.vsu.ru
It is established that UV-light (240—390 nm) in doses of 151, 1510 and 3020 J/m2 and reactive oxygen species
and singlet oxygen induce DNA fragmentation lymphocytes cells of the human 20 h after influence. Using a
method of DNA-comets it is revealed that DNA damages (single strand breaks) are found out right after UV-irradiations
of lymphocytes in doses of 1510 and 3020 J/m2 and additions hydrogen peroxide in concentration of
10-6 mol/l (a comet of type C1) and reach a maximum through 6 h after influence on of cells UV-light and ROS
(comets of types C2 and C3). Assumption about the leading part of a p53-dependent way in realization apoptosis
human lymphocytes in the conditions of influence of UV-light and reactive oxygen species is put forward.
Key words: lymphocytes, DNA-comets, apoptosis, UV-light, reactive oxygen species
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