HUMAN INTRA-INTRONIC MINISATELLITE UPS29 ASSOCIATED
WITH NEUROLOGICAL DISEASES REGULATES REPORTER GENE EGFP
EXPRESSION DEPENDING ON CELL TYPE
L. K. Sasina, N. A. Slominskaja, I. O. Suchkova, E. V. Pitsik, K. V. Solovyov,
N. A. Grudinina, T. A. Klinskaja, E. L. Patkin 1
Institute of Experimental Medicine RAMS, St. Petersburg;
1 e-mail: elp44@mail.ru
Earlier, it was established that polymorphism of minisatellite UPS29 located in one of introns of human gene
CENTB5 (ACAP3) was associated with Parkinson’s disease and epilepsy. The main aim of this work was to
elucidate if that minisatellite could regulate reporter gene activity, and if such activity was tissue (cell)-specific.
To this end there was used transient transfection of HeLa cells, mouse embryonal carcinoma line F9, and rat astrocytes
cultures with plasmides which contained reporter gene EGFP under eukaryotic promoter ROSA26 and
different allelles of minisatellite UPS29. It was found that UPS29 possessed enhancer-like activity in neuronal
type cells.
Key words: minisatellite UPS29, CENTB5, intron, enhancer, reporter gene, transient transfection, mouse
embryonal carcinoma F9, HeLa cells, rat astrocytes
| Pdf |
Back |
Contents |
Main |
