THE EFFECT OF SYNTHETIC POLYCATION POLYALLYLAMINE ON ADHESION
AND VIABILITY OF CHL V-79 RJK CHINESE HAMSTER FIBROBLASTS
WITH DIFFERENT HEAT RESISTANCE
V. P. Ivanova,1 T. M. Grinchuk,2 L. L. Alekseenko,1
I .V. Artsybasheva,1 I. I. Gavrilova 3
1 Sechenov Institute of Evolutionary Physiology and Biochemistry, RAS,
2 Institute of Cytology RAS, and
3 Institute of Macromolecular Compounds RAS, St. Petersburg;
e-mail: valet@iephb.ru
The effects of synthetic polycation polyallylamine (PAA) on adhesion of CHL V-79 RJK fibroblasts and
CHL V-79 RJK40 cells resistant to 40 °C, and attachment to these cells to polycation immobilized on polystyrene
surface were studied. We have also investigated the cytotoxicity of PAA. It was shown that cell adherence to polystyrene
plastic coated with PAA was enhanced or decreased in dependence of the PAA concentration used for
surface coating and did not depend on heat resistance of investigated cell lines. The effect of PAA on cell adhesion
to uncoated polystyrene surface after cell exposure with PAA depended not only on the polycation concentration,
but also on the extent of heat resistance of investigated cell lines. Pretreatment of CHL V-79 RJK cells
with PAA at the nontoxic concentrations led to inhibition of cell adhesion, and no change in adhesive properties
of thermoresistant cells was found under the same conditions. PAA was toxic for CHL V-79 RJK and CHL V-79
RJK40 cells only at concentrations of 100 μg/ml (MTT assay). PAA-induced acute toxicity was accompanied by
necrotic-like cell damage. Possible mechanisms of the PAA effect on the behaviour of cells with different structural
and metabolic characteristics that are due to the temperature of cell cultivation are discussed.
Key words: polyallylamine, polycation, adhesion, viability, heat resistance, fibroblasts
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