HUMAN SUBCUTANEOUS ADIPOSE TISSUE SUBJECTED TO COLD SHOCK AS A SOURCE
OF VIABLE CELLULAR POPULATION WITH CHARACTERISTICS OF MULTIPOTENT
MESENCHYMAL STROMAL CELLS
I. P. Savchenkova,1 S. V. Korjikova 2
1 Ya. R. Kovalenko All-Russian State Research Institute of Experimental Veterinary RAAS,
and 2 Institute of Stem Cell, Moscow;
e-mail: s-ip@mail.ru
Cellular population with characteristics of multipotent mesenchymal stromal cells (MMSCs) was isolated
from subcutaneous adipose tissue frozen without any cryoprotectant at –70 °N. Under critical for the adipose tissue
condition, the cells retained their viability in vitro and ability of adhesion to plastic. Cellular population was
homogeneous and represented by small cells (d — 7 μm) with fibroblast-like morphology. Cells were positively
stained with Abs for the Abs: CD29, CD44, CD49a, b, d, CD73, CD90, CD105, CD166, HLA ABC. Cells were
negative for CD34, CD45 — markers of hematopoietic cells, CD31 — marker of endothelial cells, Stro-1, as
well as for HLA DR, DP, DQ (flow cytometer analysis). Being induced to differentiate in vitro, the cells were
able to differentiate into cells similar to cells of bone, adipose and cartilage tissue. Karyological assay of the
cells isolated from human adipose tissue subjected to cold shock revealed diploid set of chromosomes, 46, XX,
without aneuploidy and structural reconstructions of chromosomes. Thus, it has been established that, under extreme
condition for the organism, the population of cells with a phenotype similar to miltipotent mesenchymal
stromal cells is preserved in subcutaneous adipose tissue.
Key words: multipotent mesenchymal stromal cells, subcutaneous adipose tissue, cold shock, viability,
cellular population, homogeneity, gene expression, differentiation
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