NUCLEAR HETEROGENEITY AND PROLIFERATIVE CAPACITY OF HUMAN
ADIPOSE DERIVED MSC-LIKE CELLS
A. V. Lavrov, S. A. Smirnikhina
Research Center for Medical Genetics RAMS, Moscow;
e-mail: avlavrov@yandex.ru
Adipose derived stem cells (ADSCs) are MSC-like cells which could be easily used for regenerative medicine.
Here, the morphology and proliferative capacity of human ADSCs is discribed. ADSCs were analyzed after
one month of cultivation at a density of 10 cells/cm2. 21 colonies were counted. Few atypical cells (huge nuclei
and cytoplasm) were found in 9 out of 17 colonies analyzed. ANOVA demonstrated that colonies also differed
(Ð =0.0025) in nuclei dimensions and scatter in the dimensions in each colony. Nuclei dimensions and cell
density logarithms correlated in reverse proportion (–0.7; Ð = 0.002). Thus, ADSCs were heterogeneous and represented
two types of cells: small highly proliferative and large low proliferative cells. Cell heterogeneity observed
in some colonies might be due to cells registered at different cell cycle phases. Stable and typical morphology,
colony-formation capability and high proliferative capacity of cells indicate visceral adipose tissue as a
rich source of ADSCs.
Key words: adipose derived stem cell, heterogeneity of mesenchymal stem cell morphology
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