AN EXTRACELLULAR Ph CHANGES REGISTRATION BY CONFOCAL MICROSCOPY
IN HIGHER PLANT AT THE EXCITATION POTENTIALS GENERATION
V. A. Vodeneev,1 E. K. Akinchits,1 L. A. Orlova,1 V. S. Sukhov,1 I. V. Balalaeva 1, 2
1 N. I. Lobachevsky State University of Nizhny Novgorod
and 2 Institute of Applied Physics RAS, Nizhny Novgorod;
1 e-mail: v.vodeneev@mail.ru
Confocal microscopy technique was applied for registration of apoplast pH changes in the Cucurbita pepo
seedling stem during generation of action potential and variation potential. Fluorescent dye FITC-dextran was
used for pH-changes registration. Analysis of fluorescence images and fluorescence spectra showed, that
FITC-dextran was localized in cell walls. Propagation of action potential and variation potential was accompanied
by transient increase of fluorescence intensity, which indicated alkalization of cell walls. This transient alkalization
is proposed to be caused by a temporary inactivation of the H+-pump in the plasma membrane.
Key words: higher plants, confocal microscopy, pH-change, excitation potentials, proton pump
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