COMPARATIVE ANALYSIS OF SGLT1 AND GLUT2 TRANSPORTER DISTRIBUTION
IN RAT SMALL INTESTINE ENTEROCYTES AND CACO2 CELLS DURING HEXOSE ABSORPTION
N. M. Grefner,1 L. V. Gromova,2 A. A. Gruzdkov,2 Ya. Yu. Komissarchik 1
1 Institute of Cytology RAS and 2 Pavlov Institute of Physiology RAS, St. Petersburg;
e-mail: grefner@mail.cytspb.rssi.ru
SGLT1 and GLUT2 hexose transporter distribution into enterocytes of small intestine isolated loop and Caco2
cell culture after absorption of high and low hexose concentrations has been considered. SGLT1 was found
along intestine villus edge in isolated loop. After high concentration hexose load GLUT2 appeared to be situated
in the apical parts of enterocytes. It is evident that GLUT2 participates in hexose transport across apical membrane.
Cultured Caco2 cells form microvilli and cell junction complex typical for enterocyte. Glucose and galactose
absorption by the cells from incubation medium has been observed. SGLT1 transporter is situated in the
apical parts and around the nuclens of Caco2 cells and combined into globules. After low concentration hexose
load, CLUT2 transporter is localized in the basal parts of Caco2 cells. Caco2 cell culture can be used as a model
for studying of hexose transport in small intestine epithelium.
Key words: confocal and electron microscopy, enterocyte, Caco2, SGLT1, GLUT2, hexose
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