N-ACETYLCYSTEINE-INDUCED REDUCTION IN SUSCEPTIBILITY OF TRANSFORMED
AND EMBRYONIC CELLS TO LYTIC ACTIVITY OF NATURAL KILLER CELLS
I. A. Gamaley,1 K. M. Kirpichnikova, E. A. Vakhromova, N. A. Filatova
Institute of Cytology RAS, St. Petersburg;
1 e-mail: igamaley@mail.cytspb.rssi.ru
We studied N-acetylcysteine (NAC) ability to change the phenotype properties of several transformed and
embryonic cells. We examined human epidermoid carcinoma A431 cells, murine hepatoma MH22a cells, and
murine embryonic fibroblasts (MEFs) in terms of the sensitivity to natural killer (NK) recognition and abolishment.
We have demonstrated that treatment with NAC (10 mM) results in a loss of susceptibility to NK cell activity
by transformed A431 and MH22a cells similar to 3T3-SV40 transformed cells whose partial reversion caused
by NAC was revealed by us before. We have shown that MEFs are also sensitive to NK activity and abolished
by NK nells as well as transformed cells. MEFs pretreated with 10 mM NAC as well as transformed cells
lose their susceptibility to NK cell activity. The loss of cell sensitivity to NK cytolytic activity was accompanied
by a reorganization of the actin cytoskeleton and the appearance of well-pronounced stress-fibers.
Key words: N-acetylcysteine, A431 cells, MH22a cells, 3T3-SV40 fibroblasts, murine embryonic fibroblasts,
natural killer cells
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