EFFECT OF IMMUNIZATION ON THE IN VIVO IMMUNOCYTES REACTION TO FOREIGN PARTICLES IN THE LARVAE OF THE FLESH FLY
CALLIPHORA VOMITORIA
T. V. Kind
Department of Entomology, Laboratory of Insect Biopharmacology and Immunology,
St. Petersburg State University;
e-mail: tatiana.kind@mail.ru
Bacterial immunization of Calliphora vomitoria larvae induces hemocytic defense reaction in response to
abiotic foreign particles injections. This reaction depends on the larval age and, consequently, the immunocytes
composition. The juvenile plasmatocytes which are abundantly present in the end feeding and crop emptying
larvae are initially very active and their reaction rate does not depend on the immunization. The plasmatocytes I
appear after crop emptying. Immunization has a positive effect on their differentiation rate and, correspondently,
the rapid progress in the defense response. The proportion of competent plasmatocytes in immunized larvae increases.
The stable hyaline cells formation also depends on immunization, although elements of this response vary
at different age steps. immunization of the end feeding and crop emptying larvae induces an increase in the
percentage of hyaline cells, but alien particles injection does not provide additional stimulating effect. After
crop emptying, immunization of wandering larvae ceases to exert an obvious direct influence, but significantly
increases the sensitivity to charcoal injection. The emergence of foreign particles in the hemolymph of immunized
wandering larvae causes a rapid increase in the number of hyaline cells due to prohemocytes and undifferentiated
plasmatocytes differentiation.
Key words: insects, Calliphora, immunity, phagocytosis, plasmatocytes, hyaline cells
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