AGGLUTINATION AND PHAGOCYTOSIS OF FOREIGN ABIOTIC PARTICLES BY BLUEBOTTLE CALLIPHORA VICINA HAEMOCYTES IN VIVO.
II. INFLUENCE OF THE PREVIOUS SEPTIC IMMUNE INDUCTION ON HAEMOCYTIC ACTIVITY
T. V. Kind
Department of Entomology, Laboratory of Insect Biopharmacology and Immunology,
St. Petersburg State University;
e-mail: tatiana.kind@mail.ru
The rate of Calliphora vicina haemocytic defense reaction to foreign particles injection depends on the larval
age and on the previous bacterial immunization. Immunization of crop-empting larvae induces an evident
increase in particles phagocytosis by juvenile plasmatocytes in 2—4 h after injection. Both the hemogram
and the pattern of cellular defense reaction change significantly after crop-empting. Immunized larvae start intensive
adhesion of foreign particles to plasmatocytes surface and formation of great aggregations of plasmatocytes
(morules) no longer than in 3—4 min after injection. The period of particle-haemocyte adhesion is
short-termed and no more than after 30 min cell aggregates dissociate and adhered charcoal particles pass to
thrombocydoidal agglutinates. Unimmunized control larvae of the same age have shown no adhesion and morules
formation. In immunized wadering and diapausing larvae, formation of capsules consisting of central thrombocydoidal
agglutinat filled with alien particles and adherent plasmatocytes I is intensified. In contrast to morules,
this capsule formation is not accompanied by charcoal particles adhesion to plasmatocytes. Immunization of
mature larvae of C. vicina shown no prominent influence on both the rate of phagocytosis and the hyaline cells
differentiation. It might be supposed that the receptors system is complex and the immunization both the mechanisms
of foreigners recognition (adhesion, morulation and incapsulation) and the far more lately occurring phagocytosis.
Key words: insects, Calliphora, immunity, phagocytosis, plasmatocytes, thrombocytoids, hyaline cells
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