2010. Vol. 52, N 6, p. 459-465
NUCLEOSOME REMODELING ON THE REGULATORY REGION OF THE RAT TRYPTOPHAN DIOXYGENASE (tdo) GENE DURING TRANSCRIPTION IN VIVO

G. I. Chikhirzhina,1,* N. U. Nazarova,1 E. V. Chikhirzhina,2 E. V. Romanovskaya 1

1 St. Petersburg State University, Department of Biochemistry,
and 2 Institute of Cytology RAS, St. Petersburg;
* e-mail: chikhirzhina@mail.ru

Two DNase I-hypersensitive regions are identified on the regulatory region of the rat tryptophan dioxygenase (tdo) gene which is expressed tissue-specifically under control of glucocorticoid hormones. DNase I-hypersensitive regions are identified in position –470 and in the vicinity of the first upstream gene. Micrococcal nuclease digestion pattern of this region shows disturbances in the regular cleavage and appearance of shorter DNA molecules than nucleosomal DNA. However the control experiments demonstrate that the same DNA region could be involved in the regular nucleosome core particles under in vitro reconstitution. Taken together, these data show that the nucleosome array in the regulatory region of the actively transcribed tdo gene in vivo is disturbed.

Key words:  the regulatory region of the rat tryptophan dioxygenase (tdo), DNase I-hypersensitive regions, micrococcal nuclease digestion pattern, nucleosome remodeling in vivo


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