CD14++CD16– AND CD14+CD16+ HUMAN MONOCYTES ADHESION TO ENDOTHELIAL CELLS
E. A. Starickova,1 A. M. Lebedeva, I. S. Freidlin
Institute of Experimental Medicine RAMS, St. Petersburg;
1 e-mail: Starickova@yandex.ru
Two subsets of monocytes were identified in humans and other mammals blood based on different levels of
CD14 and CD16 expression. These subsets have different patterns of adhesion molecules and chemokine receptors,
which suggest different modes of interaction with endothelium and tissue traffic. Here, we investigated the
ability of CD14+CD16+ and CD14++CD16– monocytes to adhesion to endothelial cells monolayer in presence
and in the absence of pro- and anti-inflammatory cytokines. We demonstrated that CD14+CD16+ monocytes had
higher level of adhesion to intact endothelial cells monolayer than CD14++CD16– monocytes. Significant increase
in adhesion of CD14++CD16– and CD14+CD16+ monocytes subpopulations was observed in the presence of
both TNFa and TNFa combinations with other cytokines. IFNa and IL-4 showed no independent effects on adhesion
of monocytes. These results have demonstrated that both CD14++CD16– and CD14+CD16+ monocytes
can be recruited to inflamed endothelium, but, in the absence of inflammation, CD14+CD16+ monocytes adhere
to endothelial cells two times stronger than CD14++CD16– monocytes.
Key words: CD14++CD16– and CD14+CD16+ monocytes subpopulations,
endothelial cells, adhesion, cytokines
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