THE EFFECT OF INHIBITORS OF SERINETHREONINE PROTEIN KINASES ON ARABIDOPSIS THALIANA ROOT MORPHOLOGY AND MICROTUBULES
ORGANIZATION IN ITS CELLS
Ya. A. Sheremet,1,* A. I. Yemets,1 K. Vissenberg,2 J.-P. Verbelen,2 Ya. B. Blume 1
1 Institute of Food Biothechnology and Genomics National Academy of Sciences of Ukraine, Kiev,
and 2 University of Antwerp, Belgium;
* e-mail: yarasheremet@gmail.com
The effect of different types of serine/thereonine protein kinases inhibitors (cyclin-dependent, Ca2+-calmodulin-
dependent and protein kinase C) on microtubules organization in cells of Arabidopsis thaliana main primary
root zones were investigated in vivo. The microtubules in epidermal and cortex cells in the transition and
elongation zones as well as microtubules in trichoblasts and atrichoblasts in the differentiation zone showed the
greatest sensitivity to protein kinases inhibitors studied. It was established that microtubules in these cell types
modified their initial transverse/oblique orientation to a chaotic or longitudinal relative to the major axis of primary
root as a result of serinethereonine protein kinases inhibition. The microtubules in cells in root meristematic
zone as well as in root hairs were less sensitive to influence of protein kinases inhibitors tested. Alterations of
microtubules orientation in the cells in primary root zones under the influence of serinethereonine protein kinases
inhibitors led to further disturbances in growth and differentiation processes. It was assumed that phosphorylation
of microtubules proteins, especially tubulin, might be involved in the regulation of these processes.
Key words: microtubules, phosphorylation, serinethereonine protein kinases, inhibitors, Arabidopsis
| Pdf |
Back |
Contents |
Main |
