DOXORUBICIN AND MENADIONE REDUCE CELL PROLIFERATION OF SACCHAROMYCES CEREVISIAE BY DIFFERENT MECHANISMS
Yu. V. Saenko, A. M. Shutov, E. V. Rastorgueva
Ulyanovsk State University;
e-mail: saenkoyv@yandex.ru
The aim of this study was to determine the impacts of doxorubicin and menadion on cell proliferation, cell
distribution in accordance with the phases of the cell-cycle phase, glutathione concentration, ribonucleotide reductase
expression, and Yap1 dependent redox-sensitive pathway activity using Saccharomyces cerevisiae as
eukaryote cell model. Our data show that menadione induced cell cycle arrest in G1-phase, reduction of intracellular
GSH, an increase in GSSG concentration, and dose-dependent increases in ribonucleotide reductase expression
and the activity of Yap1 pathway. Doxorubicin induced cell cycle arrest in G1- and S-phases, increased
GSH and GSSG concentrations, increased expression of ribonucleotide reductase, and modulated Yap-dependent
pathway activity.
Key words: doxorubicin, menadione, ribonucleotide reductase, cell cycle, Yap1
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