2010. Vol. 52, N 5, p. 420-425

CHROMOSOMAL INSTABILITY OF IN VITRO CULTURED MOUSE ES AND IPS CELLS Yu. M. Minina,1, 2 N. S. Zhdanova,1, 2 A. G. Shilov,1, 2 E. N. Tolkunova,3 M. A. Liskovykh,3 A. N. Tomilin 3 1Novosibirsk State University, 1 Institute of Cytology and Genetics Siberian Branch of RAS, Novosibirsk, and 3 Institute of Cytology RAS, St. Petersburg; e-mail: 1 minina_jul@mail.ru 3 antom@mail.cytspb.rssi.ru A perspective of using embryonic stem (ES) and induced pluripotent stem (iPS) cells in clinical medicine makes karyological analysis of these cells an important issue. Using methods of classical and molecular cytogenetics chromosomal analysis, we have carried out karyological study of two mouse ES and two iPS cell lines derived de novo. We have found monosomy of X chromosome in all studied ES and iPS cell lines, thus making a modal number of chromosomes in these cell lines 39. A chromosomal instability (aneuploidy) was revealed in both studied iPS cell lines. Moreover, we have detected chromosomal rearrangements and chromosomal fragments in one of iPS cell line. Our findings underline the importance of careful cytogenetic evaluation of pluripotent cell lines, especially iPS cell lines, which should be carried out prior to any clinical use of these cells. Key words:  embryonic stem cells, induced pluripotent stem cells, karyotype instability of ES and iPS cells, aneuploidy, monosomy of X chromosome | Pdf | Back | Contents | Main |