INFLUENCE OF PRIMARY ADHESIVE INTERACTIONS WITH FIBRONECTIN ON CLONAL GROWTH AND OSTEOGENIC POTENTIAL OF RAT MESENCHYMAL
STROMAL CELLS
O. V. Payushina,1 O. N. Khnykova, N. N. Butorina, E. I. Bueverova,
A. A. Minin, V. I. Starostin
N. K. Koltzov Institute of Developmental Biology RAS, Moscow;
1 e-mail: payushina@mail.ru
Interactions with extracellular matrix including fibronectin (Fn) play an important role in regulation of cell growth and differentiation. Influence of Fn and its individual
domains on adhesion and osteogenic potencies of rat mesenchymal stromal cells (MSCs) was estimated. Investigation of bone marrow or fetal liver MSCs adhesion
dynamics showed that after 7 days of cultivation on Fn the number of adhered clonogenic cells derived from both sources was comparable to their number observed on
plastic but their content in suspension was commonly decreased. Population of fetal liver MSCs differed from bone marrow-derived population by greater fraction of cells
that adhered for the first 7 days. Bone marrow MSC cultures on Fn were characterized by reduced activity of alkaline phosphatase as compared with cultivation on plastic;
furthermore, they deposed significantly smaller amount of calcium salts under cultivation in osteogenic medium. Cultivation of MSCs on Fn fragments demonstrated the
primary role of its cell-binding domain in the inhibition of osteogenesis.
Key words: mesenchymal stromal cells, extracellular matrix, fibronectin, adhesion, osteogenesis
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