COMPARATIVE LOCALIZATION OF CHICKEN FIVE FUNCTIONAL GENES AND THREE MICROSATELLITES ON QUAIL MITOTIC CHROMOSOMES
BY TWO-COLOR FISH-HYBRIDIZATION
A. V. Trukhina, A. F. Smirnov
Department of Genetics and Selection, St. Petersburg State University;
e-mail: trukhina_ant@mail.ru
In order to localize chicken genes and microsatellites we used two-color FISH and chicken chromosome specific BAC-clones. All BAC-clones were verified by PCR.
Analysis of the results obtained showed that: maf gene formed one linkage group with mc1r gene (CJA11), aldh1al - with igvps gene (CJA15),
pno - with acaca gene (CJA19), fzf - with bmp7 gene (CJA20), cw01 - with ubapw2w gene (CJAW). Microsatellite ADL0254 was
localized jointly with insr gene (CJA28), while LEI0342 and MCW0330 microsatellites - with hspa5 gene (CJA17). The same work was fulfilled on chicken
mitotic chromosomes. We obtained other results. maf gene was localized independently of mc1r (GGA11), aldh1al was localized independently of
igvps gene (GGA15), and pno gene (GGA19) was localized independently of acaca gene. ADL0254 and LE10342 microsa-tellites had two sites of
localization (GGA28, GGA17 accordingly and other site). Localization for genes cw01 and fzf, and for MCW0330 microsatellite was confirmed.
Key words: BAC, chicken, FISH, gene, linkage group, microsatellite, quail
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