INFLUENCE OF BIGINELLI PYRIMIDINE ON PRODUCTION REACTIVE OXYGEN SPECIES BY POLYMORPHONUCLEAR LEUKOCYTES
Yu. V. Shatalin,1,2,* V. S. Shubina,1,2 A. S. Fisyuk 3
1 Pushchino State University, 2 Institute of Theoretical and Experimental Biophysics RAS, Pyshchino,
and 3 F. M. Dostoevsky Omsk State University;
* e-mail: it@rambler.ru
The effect of 6 synthetic Biginelli pyrimidines on production of reactive oxygen species by polymorpho-nuclear neutrophils was investigated. It has been shown by
method of luminol-dependent chemiluminescence that test compounds in a concentrations of 10-100 μM stimulate production of reactive oxygen species. An
increase in the reactive oxygen species production by stimulated neutrophils in the presence of 10 μM 1-(3, 4-di-metoxyphenyl)ethyl-4(alkyl/aryl) substituted Biginelli
pyrimidines was 50-90 %. An increase in the priming effect of Biginelli pyrimidines on reactive oxygen species production by neutrophils was noted in the case of replacement
of phuryl radical by phenyl and alkyl radicals at C(4) pyrimidine cycle and in the case of replacement benzyl radical at N(1) by 3, 4-dimetoxyphenyl radical. It has been
revealed a high inhibitory effect of 1-(3, 4-dimetoxyphenylethyl)-4(alkyl/aryl) substituted Biginelli pyrimidine in concentration 0.01-0.10 ìM. It has been found that high
concentration of ethyl-1-[2-(3, 4-dimethoxyphenyl)ethyl]-6- methyl-4-phenyl-2-thioxo-1, 2, 3, 4-tetrahydropyrimidine -5-carboxylate (1 mM and more) is able to cause respiratory
burst of neutrophils without additional stimulation.
Key words: neutrophils, reactive oxygen species production, Biginelli pyrimidine, purine (pyrimidine) receptors
| Pdf |
Back |
Contents |
Main |
