CULTIVATED HUMAN HAIR FOLLICLE CELLS ARE CAPABLE OF INTEGRATING TO SKIN STRUCTURE IN VIVO
E. S. Chermnykh,1,* N. V. Radyukhina,2 P. N. Rutkevich,2 A. Y. Shevelyov,2 T. N. Vlasik,2
E. A. Vorotelyak,1 A. V. Vasiliev,1 V. V. Terskikh 1
1 1 N. K. Koltzov Institute of Developmental Biology RAS and 2 Institute of Experimental Cardiology, Cardiology
Research-and-Production Complex, Federal Agency for Health Care and Social Development, Moscow;
* e-mail: elinachermnykh@mail.ru
In the present study, human keratinocytes and dermal papilla cells were labeled to investigate their behaviour after intradermal transplantation. Cells were transduced by
lentiviral vectors that bore marker gene encoding green fluorescent protein (copGFP) or red fluorescent protein (DsRed). A portion of transgene expressing cells was evaluated
by flow cytometry. Genetic constructions that we used provided high level (> 95 %) of transducti-on of hair follicle cells. In vitro transduced cells were injected under the
epidermis of human skin fragments, and these fragments were then transplanted under the skin of immunodeficient mice. Injected epidermal keratinocy-tes were found,
mainly, in hair follicles and partially in a zone of interfollicular epidermis, while dermal papilla cells were found in papilla derma. The results of the present research show that
the chosen genetic constructions obtained on a basis of human immunodeficiency lentivirus are capable of effective and stable transduction of human skin cells. Injected cells
survived and were found in the corresponding structures of the skin.
Key words: dermal papilla cells, keratinocytes, lentivirus vectors, transduction
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