ANALYSIS OF CHROMOSOME COMPOSITION IN INTERSPECIFIC EMBRYONIC STEM HYBRID CELLS OF MICE
I. E. Pristyazhnyuk,1 N. M. Matveeva, A. S. Graphodatsky,
N. A. Serdukova, O. L. Serov
Institute of Cytology and Genetics, Siberian Branch of RAS, Novosibirsk;
1 e-mail: iprist@ngs.ru
Chromosome complements of twenty hybrid clones obtained by fusion of Mus musculus embryonic stem cells (ESC) and M. caroli splenocytes were
studied. Using of double-color in situ hybridization with chromosome- and species-specific probes we were able to detect the parental origin for each chromosome in
hybrid cells. Based on parental chromosome ratio, all 20 hybrid clones were separated in some different groups: from the group containing practically tetraploid M.
musculus genome with single M. caroli chromosomes to hybrids with dominance of M. caroli chromosome homologues. In 8 hybrid cells clones we
observed prevalence of chromosomes originated from ESC in ratio from 5 : 1 to 3 : 1. Another hybrid cells clones have either equal (1 : 1, 1 : 2) ratio of M. musculus
to M. caroli chromosomes or with the prevalence of ESC- (2 : 1) or splenocyte- (1 : 2) originated parental chromosome homologues. In 3 hybrid cells clones, we
observed preferable segregation of ESC-originated pluripotent chromosomes. This phenomenon was found for the first time and it possibly indicates compensation of the
epigenetic differences between parental chromosomes of ESC- and splenocyte-origination.
Key words: embryonic stem hybrid cells, double-color in situ hybridization, chromosome homologues, ploidy, segregation of chromosomes
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